Areas of special interest and accomplishments
Canadian Medical Research Fellowship
The I.U.P.H.A.R. Fellowship
Established Investigator of the American Heart Association
Elected Chairman of the FASEB Smooth Muscle Conference for 1994
Invited reviewer on "Excitation-Contraction Coupling in Smooth
Muscle" by Physiological Review.
Appointed to the NIH Parent Review Committee for the Program Project
applications in Vascular Biology and Medicine.
Director of the Vancouver Vascular Biology Research Centre
My research interests focus on the control of blood
vessel function in health and disease. My specialty is Ca2+ signaling
in smooth muscle and endothelial cells. Two intriguing questions in
vascular Ca2+ signaling are: 1) How does one signaling ion "Ca2+
" control a number of separate functions in the same cell and 2)
What are the ionic mechanisms responsible for vascular heterogeneity?
Our working hypothesis is that the informational content of Ca2+ signaling
is greatly enhanced by the ultrastructural alignment of different ionic
transport molecules in the membranes of the ER, nuclear envelope, Golgi
apparatus, mitochondria and the cell surface to cause local cytoplasmic
Ca2+ gradients and oscillations. Our laboratory utilizes confocal microscopy
combined with measurements of force and membrane currents and potentials
to elucidate the mechanisms of intracellular Ca2+-signaling in smooth
muscle and endothelial cells. In addition we are interested in relating
changes in cell signaling in response to pathological insults to early
functional changes in the development of vascular disease.
Education / Training:
Alberta Research Council
Clinical Veterinary Medicine 1960-1961
University of Toronto DVM Ontario Veterinary
University of Alberta MSc Department
of Physiology 1962
University of Alberta PhD Department
of Pharmacology 1965
Karkan, D.M., van Breemen, C., Skarsgard, P.L., Lagaud,
G.J., Hardwick, D.F. A
link between vasomotion and spontaneous oscillations of oxygen in rat
brain. Ad. Exp. Med. Biol. 471:111-6, 1999.
Whitehurst, R.M., Laskey, R., Goldberg, R.N., Herbert,
D., van Breemen, C. Influence
of group B streptococci on piglet pulmonary artery response to bradykinin.
J. Appl. Physiol. 86(1):61-5, 1999.
Lagaud, G.J., Lam, E., Lui, A., van Breemen, C., Laher,
inhibition of myogenic tone by PD98059, a MEK1 inhibitor, in rat middle
cerebral arteries. Biochem. Biophysiol. Res. Comm. 257(2):523-7,
Wang, X., van Breemen, C. Depolarization-mediated
inhibition of Ca(2+) entry in endothelial cells. Am. J. Physiol.
277(4 Pt 2):H1498-504, 1999.
Lagaud, G.J., Skarsgard, P.L., Laher, I., van Breemen,
of endothelium-dependent vasodilation in pressurized cerebral and small
mesenteric resistance arteries of the rat. J. Pharmacol. Exp. Ther.
Li, L., Bressler, B., Prameya, R., Dorovini-Zis, K.,
van Breemen, C. Agonist-stimulated
calcium entry in primary cultures of human cerebral microvascular endothelial
cells. Microvasc. Res. 57(3):211-26, 1999.
Skarsgard, P.L., Wang, X., McDonald, P., Lui, A.H.,
Lam, E.K., McManus, B.M., van Breemen, C., Laher, I. Profound
inhibition of myogenic tone in rat cardiac allografts is due to eNOS-
and iNOS-based nitric oxide and an intrinsic defect in vascular smooth
muscle contraction. Circulation. 101(11):1303-10, 2000.
Ruehlmann, D.O., Lee, C.H., Poburko, D., van Breemen,
C. Asynchronous Ca(2+) waves in intact venous smooth muscle. Circ. Res.
Wang, X., Kim, S.U., van Breemen, C., McLarnon, J.G.
of purinergic P2x receptors inhibits P2y mediated Ca2+influx in human
microglia. Cell Calcium 27(4):205-212, 2000.
Granville, D.J., Cassidy, B.A.,
Ruehlmann, D., Choy, J., Brenner, C., Kroemer, G., Margaron, P., van
Breemen, C., Hunt, D.W. and McManus, B.M. Mitochondrial
Release of Apoptosis Inducing Factor (AIF) and Cytochrome c During Smooth
Muscle Cell Apoptosis. Am J Path 159:305-311, 2001.